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New Molecular Diagnostics Platform Enables Rapid Detection of BRAF V600 Mutations

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Key Points

  • The new molecular diagnostics prototype can detect BRAF V600 mutations in melanoma in about 90 minutes and requires no sample preparation.
  • The diagnostic platform was 95% in concordance with the standard method approved by clinical laboratory improvement amendments.

A new diagnostic platform to detect BRAF mutations in melanoma and other cancer types is faster and more accurate compared with the standard method currently used in clinics, and this could help accelerate diagnosis and treatment, according to results presented at the AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics, held October 19 to 23 in Boston (Abstract C198).

New Molecular Diagnostics Platform

The new molecular diagnostics prototype platform developed by Biocartis in Mechelen, Belgium, can detect BRAF V600 mutations in about 90 minutes, requires no sample preparation, and is 95% in agreement with the standard method approved by clinical laboratory improvement amendments (CLIA).

“When you do molecular testing for BRAF mutations the way it is done in the clinic, it often takes 3 to 4 weeks to get the results back. A lot of that time is spent preparing the sample, including laser microdissection to isolate DNA from the tumor cells,” said Filip Janku, MD, PhD, an oncologist at The University of Texas MD Anderson Cancer Center in Houston. “With the [molecular diagnostics] platform, it takes about 2 minutes to add fresh, frozen, or paraffin-embedded tumor samples to the sample cartridge and less than 90 minutes to get the results. This platform is capable of giving you an answer in an absolutely unprecedented time frame.

“We used [molecular diagnostics] to detect BRAF mutations as a proof of principle. This new platform will be capable of detecting a variety of mutations, including KRAS, EGFR, and others for which there are FDA-approved targeted therapies,” he added. “Unfortunately, we are still not in a position to offer targeted therapies to patients as soon as they come to the clinic, because it takes time to do molecular testing. Identifying the right treatment for patients early is crucial in cancer care. We need to invest more in developing assays and diagnostic platforms.”

Study Details

Dr. Janku and colleagues used 79 archival tumor samples to test the molecular diagnostics platform. BRAF mutation status obtained using the CLIA-approved method was available for all these samples. The results generated using the new platform and CLIA-approved method were in agreement for 75 of the 79 samples. Of the four samples with discrepancies, the molecular diagnostics platform but not the CLIA-approved method detected a BRAF V600E mutation in a colon cancer sample, and the molecular diagnostics platform did not detect BRAF mutations in the remaining three samples that were reported to be BRAF-mutation positive by the CLIA-approved method.

Of interest, two of the three patients whose tumor samples tested positive for BRAF mutations by the CLIA-approved method but not the molecular diagnostics platform were treated with BRAF/MEK inhibitors based on the CLIA results, and they did not respond to treatment.

This study was funded by Biocartis.

Dr. Janku reported no potential conflicts of interest.

The content in this post has not been reviewed by the American Society of Clinical Oncology, Inc. (ASCO®) and does not necessarily reflect the ideas and opinions of ASCO®.


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